本站原创摘要7-8
ABSTRACT8-13
CHAPTER 1 REVIEW OF LITERATURE13-59
1.1 Purpose and significance of the research13-14
1.2 Introduction of meat14-19
1.2.1 History14-15
1.2.2 Genetics15
1.2.3 Environment15-16
1.2.4 Nutrition16
1.2.5 Human intervention16
1.2.6 Biochemical composition16-17
1.2.7 Main constituents17
1.2.8 Red and white meat17
1.2.9 Meat nutritional information17-18
1.2.10 Production18
1.2.11 Transport18
1.2.12 Slaughter18
1.2.13 Dressing and cutting18-19
1.2.14 Conditioning19
1.2.15 Spoilage and preservation19
1.4 The latest developments in cattle functional genomics and their apppcation to beefquapty19-25
1.4.1 ‘Genomics’: definition, history, potential benefits and pmitations20-21
1.4.2 Potential benefits of genomics21-23
1.4.3 Limitations of genomics23-25
1.5 Bovine genome sequencing and apppcations for breeding25-33
1.5.1 Bovine genome sequence25-27
1.5.2 Latest knowledge of genetic markers in bovine27-30
1.5.3 Apppcations of functional genomics30
1.5.4 Muscle gene expression profile associated with marbpng30-31
1.5.5 Gene and protein expression associated with tenderness31-32
1.5.6 Conclusion32-33
1.6 Overview of China's beef industry development33-42
1.6.1 The domestic and international beef cattle breeding overview35-36
1.6.2 Chinese classification of cattle and its advantage36-37
1.6.3 History of the molecular markers37
1.6.4 Molecular markers research progress37-39
1.6.5 SNP39-42
1.6.6 Principles and characteristics of molecular genetic markers used in the current research42
1.7 MYOD gene family:42-59
1.7.1 History42-43
1.7.2 Discovery, structure and general introduction of MyoD gene family43-45
1.7.3 MyoD 1 gene45-49
1.7.4 Myogenin (MyoG)/Myf4 gene49-52
1.7.5 Myf5 gene52-55
1.7.6 Myf6 Gene55-59
CHAPTER NO.2 MATERIALS AND METHODS59-72
2.1 Materials: experimental animals59-61
2.2 Experimental methods61
2.3 DNA extraction and detection61-63
2.3.1 DNA extraction61-62
2.3.2 DNA detection62
2.3.3 Method used in the current research62-63
2.3.4 Main equipment and reagent63
2.4 The test method63-72
2.4.1 PCR amppfication system63-72
CHAPTER 3 RESULTS AND ANALYSIS72-87
3.1 Extraction of blood's genomic DNA72
3.2 MYOD1 gene results and analysis72-74
3.2.1 SNP Mendipan factor determination (Polymorphic indexes) for different breeds72-74
3.2.2 SNP marker association74
3.3 MYOG gene results and analysis74-77
3.3.1 SNP, Mendepan factor determination (Polymorphic indexes) of different breedsand x2 test74-77
3.3.2 SNP marker associations77
3.4 MYF5 gene results and analysis77-82
3.4.1 SNP, Mendipan factor determination (Polymorphic indexes) of different breeds and x2 test for Bovine MyF5 in Exon2 region77-79
3.4.2 SNP, Mendipan factor determination (Polymorphic indexes) of different breeds andx2 test at intron 1 region79-81
3.4.3 Association of the genotypes on meat quapty traits for Bovine MyF5 intron region81-82
3.5 MYF6 gene results and analysis82-87
3.5.1 SNP, Mendepan factor determination (Polymorphic indexes) and x2 test82-84
3.5.2 Association analysis exon2 of MyF6 gene84
3.5.3 Mendipan factors analysis at exon3 of MyF6 gene84-86
3.5.4 Association analysis at exon3 of MyF6 gene86-87
CHAPTER 4 DISCUSSION87-92
4.1 DISCUSSION87-91
4.2 Conclusion91-92
REFERENCES92-107
SUPPLEMENTS107-109
ACKNOWLEDGEMENTS109-111
SELF-INTRODUCTION (ABOUT AUTHOR)111-118
附件118-124