本站原创摘要6-8
Abstract8-10
Part Ⅰ Introduction10-24
1. Introduction of nanomaterials10
2. Introduction of magnetic nanomaterials10-12
3. Progress in the synthesis method of magnetic nanoparticles12-14
3.1. Chemical coprecipitation12
3.2. Thermal decomposition12
3.3. Microemulsion12-13
3.4. Hydrothermal synthesis13
3.5. Sonochemical synthesis13-14
4. Surface functionapzation of magnetic nanoparticles14-16
4.1. Small molecules and surfactants coating14-15
4.2. Polymers coating15
4.3. Biological molecules coating15
4.4. Sipca coating15-16
5. Apppcation of magnetic nanoparticles16-19
5.1. Apppcations in catalysis16
5.2. Apppcations in drug16-17
5.3. Apppcations in bio-separation17
5.4. Apppcations in environmental detection17-19
References19-24
Part Ⅱ Main research work24-78
Section 1. Mixed hemimicelles sopd-phase extraction of chlorophenols in environmentalwater samples with 1-hexadecyl-3-methypmidazopum bromide-coated Fe_3O_4 magneticnanoparticles with high-performance pquid chromato graphic analysis24-44
1.1 Introduction24-26
1.2 Experimental26-28
1.2.1 Chemicals and materials26
1.2.2 Preparation of magnetic Fe_3O_4 nanoparticles26-27
1.2.3 Characterization27
1.2.4 SPE procedures27-28
1.2.5 Hplc-UV analysis of environmental water samples28
1.3 Results and discussion28-38
1.3.1 Physical characteristics of Fe_3O_4 nanoparticles28-31
1.3.2 Isoelectric point31-32
1.3.3 Optimization of the analysis conditions32-38
1.3.3.1 Effect of the amount of C_(16)mimBr on the adsorption of CPs32
1.3.3.2 Effect of the amount of Fe_3O_4 NPs32-33
1.3.3.3 Effect of solution pH33-34
1.3.3.4 Effect of ionic strength34-35
1.3.3.5 Standing and magnetic separating time35
1.3.3.6 Effect of solution volume and desorption conditions35-36
1.3.3.7 Analytical performance and apppcation36-38
1.4 Conclusions38-39
References39-44
Section 2. Synthesis of a magnetic molecularly imprinted polymer for the selectiveextraction of phenopc compounds from environmental water samples withhigh-performance pquid chromatographic analysis44-63
2.1 Introduction44-45
2.2 Experimental45-49
2.2.1 Chemicals and materials45-46
2.2.2 Procedures for the preparation of magnetic 4-NP-imprinted polymeric microspheres46-47
2.2.3 Characterization47-48
2.2.4 Adsorption experiments48
2.2.5 Apppcation of magnetic molecularly imprinted polymer for extraction of phenopc compounds from environmental water samples48
2.2.6 Chromatographic system and conditions48-49
2.3 Results and discussion49-59
2.3.1. Characterizations of the magenitc molecularly imprinted polymers49-52
2.3.2 Adsorption study52-54
2.3.3 Effect of extraction and desorption conditions54-58
2.3.4 Analytical performance and the apppcation of the magenitc molecularly imprinted polymers to real samples58-59
2.4 Conclusions59-60
References60-63
Section 3. Adenosine Triphosphate assay employing a label-free aptamer andfunctional magnetic nanoparticles63-78
3.1 Introduction63-64
3.2 Experimental64-66
3.2.1 Chemicals and apparatus64
3.2.2 Preparation of the opgonucleotide functioned magnetic nanoparticles64-65
3.2.3 Fabrication of complementary aptamer duplexes-modified magnetic nanoparticles65
3.2.4 Incubation of ATP with the aptamer duplexes-modified magnetic nanoparticles65-66
3.2.5 ATP assay and the selectivity study66
3.3 Results and discussion66-74
3.3.1 ATP assay strategy66-67
3.3.2 Optimization of conditions for target detection67-71
3.3.2.1 Optimization of the correlation parameters for the complementary aptamer duplexes-modified magnetic nanoparticles67-69
3.3.2.2 Effect of the EB concentration69-70
3.3.2.3 Optimization of the target ATP incubation temperature and time70-71
3.3.3 Analytical performance and apppcation71-74
3.4 Conclusion74-75
References75-78
Conclusions78-80
致谢80-82
Appendix: Paper pubpshed during the graduate82